ARG40273
anti-GLUT3 antibody
anti-GLUT3 antibody for Flow cytometry,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GLUT3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Rb |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GLUT3 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide between aa. 432-463 of Human GLUT3. |
| Conjugation | Un-conjugated |
| Alternate Names | Solute carrier family 2, facilitated glucose transporter member 3; GLUT-3; Glucose transporter type 3, brain; GLUT3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | C6 | ||||||
| Observed Size | ~ 50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SLC2A3 |
| Gene Full Name | solute carrier family 2 (facilitated glucose transporter), member 3 |
| Function | Facilitative glucose transporter. Probably a neuronal glucose transporter. [UniProt] |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. [UniProt] |
| Calculated MW | 54 kDa |
Images (2) Click the Picture to Zoom In
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ARG40273 anti-GLUT3 antibody WB image
Western blot: 20 µg of C6 cell lysate stained with ARG40273 anti-GLUT3 antibody at 1:2000 dilution.
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ARG40273 anti-GLUT3 antibody FACS image
Flow Cytometry: U-2 OS cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by ARG40273 anti-GLUT3 antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by incubation with DyLight® 488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG1 (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
