ARG65553
anti-GRAP2 / GADS antibody [UW40]
anti-GRAP2 / GADS antibody [UW40] for Flow cytometry,Immunoprecipitation,Western blot and Human
Cancer antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [UW40] recognizes GRAP2 / GADS |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IP, WB |
| Specificity | The mouse monoclonal antibody UW40 recognizes GRAP2/GADS, a 41 kDa cytoplasmic adaptor protein that plays a pivotal role during the early events of signal transduction in T cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | UW40 |
| Isotype | IgG2a |
| Target Name | GRAP2 / GADS |
| Antigen Species | Human |
| Immunogen | GST-fusion human GRAP2/GADS protein |
| Conjugation | Un-conjugated |
| Alternate Names | Adapter protein GRID; Growth factor receptor-binding protein; GRPL; Protein GADS; Hematopoietic cell-associated adapter protein GrpL; Mona; GRB2L; Grf40; Grf-40; GRAP-2; GRB2-related adapter protein 2; P38; SH3-SH2-SH3 adapter Mona; GRID; Grf40 adapter protein; GRBLG; GrbX; GRB-2-like protein; GRBX; GADS |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GRAP2 |
| Gene Full Name | GRB2-related adaptor protein 2 |
| Background | GRAP2/GADS (Grb2-related adaptor protein 2 / Grb2-related adaptor downstream of Shc) is a cytoplasmic adaptor protein containing N- and C-terminal SH3 domains flanking a central SH2 domain and a proline/glutamine-rich region. It is expressed predominantly in lymphoid tissue and hematopoietic cells, particularly in T cells. GRAP2/GADS plays a pivotal role during the early events of T cell signal transduction by recruiting the adaptor protein SLP-76 and its associated molecules, such as Vav, Nck, Itk, and ADAP, to the transmembrane adaptor protein LAT. GRAP2/GADS also binds several other signaling proteins, namely Gab2, HPK1 (hematopoietic progenitor kinase 1), and Cbl. Unlike similar adaptor protein Grb2, GRAP2/GADS shows higher selectivity when binding to the particular phosphorylated tyrosines of LAT adaptor. |
| Function | Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc. [UniProt] |
| Research Area | Cancer antibody; Signaling Transduction antibody |
| Calculated MW | 38 kDa |
Clone References
The Gads (GrpL) adaptor protein regulates T cell homeostasis.
CD95/Fas induces cleavage of the GrpL/Gads adaptor and desensitization of antigen receptor signaling.
