ARG57042
anti-GRP94 antibody [94B9]
anti-GRP94 antibody [94B9] for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [94B9] recognizes GRP94 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 94B9 |
| Isotype | IgG2b, kappa |
| Target Name | GRP94 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 22-803 of Human GPR94. |
| Conjugation | Un-conjugated |
| Alternate Names | Tumor rejection antigen 1; gp96 homolog; Endoplasmin; 94 kDa glucose-regulated protein; HEL-S-125m; GP96; GRP94; TRA1; HEL35; GRP-94; Heat shock protein 90 kDa beta member 1; ECGP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSP90B1 |
| Gene Full Name | heat shock protein 90kDa beta (Grp94), member 1 |
| Background | This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012] |
| Function | Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. [UniProt] |
| Calculated MW | 92 kDa |
Images (3) Click the Picture to Zoom In
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ARG57042 anti-GRP94 antibody [94B9] ICC/IF image
Immunoflorescense: U87MG cell line stained with ARG57042 anti-GRP94 antibody [94B9] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57042 anti-GRP94 antibody [94B9] FACS image
Flow Cytometry: U87MG cell line stained with ARG57042 anti-GRP94 antibody [94B9] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57042 anti-GRP94 antibody [94B9] WB image
Western blot: 1) 25 ng of Recombinant Human GRP94, 40 µg of 2) HeLa cell lysate, 3) Jurkat cell lysate, 4) HepG2 cell lysate stained with ARG57042 anti-GRP94 antibody [94B9] at 1:3000.
