ARG22214
anti-GRP94 antibody [9G10]
anti-GRP94 antibody [9G10] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Chicken,Dog,Guinea pig,Hamster,Horse,Monkey,Pig,Rabbit,Sheep,Xenopus laevis
Overview
| Product Description | Rat Monoclonal antibody [9G10] recognizes GRP94 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Chk, Dog, Gpig, Hm, Hrs, Mk, Pig, Rb, Sheep, Xenopus laevis |
| Tested Application | FACS, ICC/IF, IP, WB |
| Specificity | Detects ~98kDa. Does not detect human Hsp90, Grp74, or GrpE from E.coli. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | 9G10 |
| Isotype | IgG2a |
| Target Name | GRP94 |
| Antigen Species | Chicken |
| Immunogen | Purified GRP94 isolated from chicken oviducts |
| Conjugation | Un-conjugated |
| Alternate Names | Tumor rejection antigen 1; gp96 homolog; Endoplasmin; 94 kDa glucose-regulated protein; HEL-S-125m; GP96; GRP94; TRA1; HEL35; GRP-94; Heat shock protein 90 kDa beta member 1; ECGP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSP90B1 |
| Gene Full Name | heat shock protein 90kDa beta (Grp94), member 1 |
| Background | This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012] |
| Function | Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. [UniProt] |
| Cellular Localization | Endoplasmic Reticulum, Endoplasmic reticulum lumen, Melanosome |
| Calculated MW | 92 kDa |
Images (3) Click the Picture to Zoom In
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ARG22214 anti-GRP94 antibody [9G10] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22214 anti-GRP94 antibody [9G10] (yellow) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
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ARG22214 anti-GRP94 antibody [9G10] WB image
Western blot: Human HeLa cell lysates stained with ARG22214 anti-GRP94 antibody [9G10] at 1:1000 dilution.
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ARG22214 anti-GRP94 antibody [9G10] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22214 anti-GRP94 antibody [9G10] (red) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
