ARG62998
anti-GST antibody [S-tag-05]
anti-GST antibody [S-tag-05] for Immunoprecipitation,Western blot and Other
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [S-tag-05] recognizes GST |
|---|---|
| Tested Reactivity | Other |
| Tested Application | IP, WB |
| Specificity | The clone S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibody is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S-tag-05 |
| Isotype | IgG2b |
| Target Name | GST |
| Antigen Species | Others |
| Immunogen | GST fusion protein |
| Conjugation | Un-conjugated |
| Alternate Names | Glutathione S-transferase kappa 1; hGSTK1; GST; GST class-kappa; GST13-13; EC 2.5.1.18; Glutathione S-transferase subunit 13; GST13; GST 13-13; GSTK1-1 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Under reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||
| Positive Control | WB: GST fusion protein. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | GSTK1 |
|---|---|
| Gene Full Name | glutathione S-transferase kappa 1 |
| Background | The GST (Glutathione-S-Transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins. |
| Function | Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB). [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
Images (1) Click the Picture to Zoom In
-
ARG62998 anti-GST antibody [S-tag-05] WB image
Western blot: Affinity purified GST from bacterial cell lysate stained with ARG62998 anti-GST antibody [S-tag-05].
