ARG55372
anti-GST3 / GST pi antibody
anti-GST3 / GST pi antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GST3 / GST pi |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GST3 / GST pi |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 97-126 (Center) of Human GST3 / GST pi. |
| Conjugation | Un-conjugated |
| Alternate Names | DFN7; EC 2.5.1.18; GST3; GSTP; GSTP1-1; HEL-S-22; FAEES3; GST class-pi; PI; Glutathione S-transferase P |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | K562 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GSTP1 |
| Gene Full Name | glutathione S-transferase pi 1 |
| Background | Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008] |
| Function | Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. [UniProt] |
| Cellular Localization | Cytoplasm. Mitochondrion. Nucleus. Note=The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 23 kDa |
Images (4) Click the Picture to Zoom In
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ARG55372 anti-GST3 / GST pi antibody ICC/IF image
Immunofluorescence: A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG55372 anti-GST3 / GST pi antibody (green) at 1:25 dilution, 1 hour at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 hour at 37°C). DAPI (blue) for nuclear staining.
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ARG55372 anti-GST3 / GST pi antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colon carcinoma stained with ARG55372 anti-GST3 / GST pi antibody.
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ARG55372 anti-GST3 / GST pi antibody WB image
Western blot: 35 µg of K562 cell lysate stained with ARG55372 anti-GST3 / GST pi antibody.
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ARG55372 anti-GST3 / GST pi antibody FACS image
Flow Cytometry: Ramos cells stained with ARG55372 anti-GST3 / GST pi antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
