ARG45894

anti-GTF2A2 antibody

anti-GTF2A2 antibody for Western blot,Flow cytometry and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes GTF2A2
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GTF2A2
Antigen Species Human
Immunogen Recombinant protein containing to human GTF2A2.
Conjugation Un-conjugated
Alternate Names GTF2A2; TF2A2; Transcription initiation factor IIA subunit 2; General transcription factor IIA subunit 2; TFIIA p12 subunit; TFIIA-12; TFIIAS; Transcription initiation factor IIA gamma chain; TFIIA-gamma

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 17 kDa

Properties

Form Liquid
Purification Affinity chromatography purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 235459 Mouse GTF2A2

GeneID: 2958 Human GTF2A2

GeneID: 83828 Rat GTF2A2

Gene Symbol GTF2A2
Gene Full Name General Transcription Factor IIA Subunit 2
Background Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).[supplied by OMIM, Mar 2008]
Function TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 12 kDa