ARG53062
anti-Galectin 1 antibody [SP247]
anti-Galectin 1 antibody [SP247] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Cell Death antibody
Overview
| Product Description | Rabbit Monoclonal antibody [SP247] recognizes Galectin 1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SP247 |
| Isotype | IgG |
| Target Name | Galectin 1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide near the C-terminus of the Human Galectin 1 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | HBL; Gal-1; GAL1; HPL; Lectin galactoside-binding soluble 1; Galectin-1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; GBP; Beta-galactoside-binding lectin L-14-I; Putative MAPK-activating protein PM12; 14 kDa lectin; Galaptin; S-Lac lectin 1; HLBP14 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Incubation Time: 10 at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Placenta, Tonsil, NIH/3T3 Cell Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A/G |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Galectin-1 (gal-1) is expressed intracellularly and extracellularly by various normal and pathological tissues. It shows a wide range of biological activity such as apoptosis, cell proliferation and cell differentiation. Gal-1 is overexpressed in endothelial cells of different human tumors, tumor cells, and extracellular matrix. It binds its ligands such as beta-galactoside and a wide array of complex carbohydrates to regulate immune responses. |
| Cellular Localization | Cytoplasm, Extracellular Matrix, Nucleus |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody |
| Calculated MW | 15 kDa |
Images (4) Click the Picture to Zoom In
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ARG53062 anti-Galectin 1 antibody [SP247] WB image
Western blot: 30 µg of A549 cell lysate stained with ARG53062 anti-Galectin 1 antibody [SP247] at 1:100 dilution.
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ARG53062 anti-Galectin 1 antibody [SP247] IHC-P image
Immunohistochemistry: Human Placenta stained with ARG53062 anti-Galectin 1 antibody [SP247].
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ARG53062 anti-Galectin 1 antibody [SP247] WB image
Western blot: NIH/3T3 cell lysate stained with ARG53062 anti-Galectin 1 antibody [SP247].
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ARG53062 anti-Galectin 1 antibody [SP247] WB image
Western blot: 30 µg of Mouse kidney lysate stained with ARG53062 anti-Galectin 1 antibody [SP247] at 1:100 dilution.
Customer's Feedback
Good
Review for anti-Galectin 1 antibody [SP247]
Application：WB
Sample：Rat kidney
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-Galectin 1 antibody [SP247]
Application：WB
Sample：Mouse kidney
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
