ARG43043
anti-Glut9 antibody
anti-Glut9 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Glut9 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Glut9 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 262-297 of Human Glut9. (EKHNEARAVKAFQTFLGKADVSQEVEEVLAESRVQR) |
| Conjugation | Un-conjugated |
| Alternate Names | Solute carrier family 2, facilitated glucose transporter member 9; GLUT-9; Glucose transporter type 9; UAQTL2; GLUT9; GLUTX; URATv1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min or EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NRM0 Human Solute carrier family 2, facilitated glucose transporter member 9 |
|---|---|
| Gene Symbol | SLC2A9 |
| Gene Full Name | solute carrier family 2 (facilitated glucose transporter), member 9 |
| Background | This gene encodes a member of the SLC2A facilitative glucose transporter family. Members of this family play a significant role in maintaining glucose homeostasis. The encoded protein may play a role in the development and survival of chondrocytes in cartilage matrices. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Urate transporter, which may play a role in the urate reabsorption by proximal tubules (PubMed:18327257, PubMed:28083649). Does not transport glucose, fructose or galactose (PubMed:28083649). [UniProt] |
| Cellular Localization | Isoform 1: Basolateral cell membrane; Multi-pass membrane protein. Isoform 2: Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. [UniProt] |
| Calculated MW | 59 kDa |
Images (6) Click the Picture to Zoom In
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ARG43043 anti-Glut9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human renal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43043 anti-Glut9 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43043 anti-Glut9 antibody WB image
Western blot: HepG2 and A549 whole cell lysates stained with ARG43043 anti-Glut9 antibody at 0.5 µg/ml dilution.
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ARG43043 anti-Glut9 antibody FACS image
Flow Cytometry: U937 cells were blocked with 10% normal goat serum and then stained with ARG43043 anti-Glut9 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG43043 anti-Glut9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43043 anti-Glut9 antibody at 2 µg/ml dilution, overnight at 4°C. The section was counterstained with DAPI (blue).
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ARG43043 anti-Glut9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43043 anti-Glut9 antibody at 2 µg/ml dilution, overnight at 4°C. The section was counterstained with DAPI (blue).
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ARG43043 anti-Glut9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestinal tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43043 anti-Glut9 antibody at 2 µg/ml dilution, overnight at 4°C. The section was counterstained with DAPI (blue).
