ARG57166
anti-Glutathione Synthetase antibody [12F2]
anti-Glutathione Synthetase antibody [12F2] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [12F2] recognizes Glutathione Synthetase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 12F2 |
| Isotype | IgG1, kappa |
| Target Name | Glutathione Synthetase |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-474 of Human Glutathione Synthetase |
| Conjugation | Un-conjugated |
| Alternate Names | Glutathione synthase; GSH synthetase; EC 6.3.2.3; HEL-S-64p; Glutathione synthetase; GSHS; HEL-S-88n; GSH-S |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GSS |
| Gene Full Name | glutathione synthetase |
| Background | Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008] |
| Calculated MW | 52 kDa |
Images (1) Click the Picture to Zoom In
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ARG57166 anti-Glutathione Synthetase antibody [12F2] WB image
Western blot: 40 µg of 1) MDA-MB231, 2) WiDr, 3) SW480, 4) A427, 5) MCF7, 6) 293T, 7) HeLa, 8) HT1080, 9) U87MG, and 10) A549 cell lysates stained with ARG57166 anti-Glutathione Synthetase antibody [12F2] at 1:1000.
