ARG64362
anti-HADH / HADHSC antibody
anti-HADH / HADHSC antibody for Western blot and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes HADH / HADHSC |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HADH / HADHSC |
| Antigen Species | Human |
| Immunogen | C-YERGDASKEDID |
| Conjugation | Un-conjugated |
| Alternate Names | Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; HAD; EC 1.1.1.35; HADHSC; HHF4; HCDH; SCHAD; Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HADH1; MSCHAD |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q16836 Human Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial |
|---|---|
| Background | This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15. [provided by RefSeq, May 2010] |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 34 kDa |
| PTM | Succinylation at Lys-81, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity). |
Images (2) Click the Picture to Zoom In
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ARG64362 anti-HADH / HADHSC antibody WB image
Western Blot: Human Kidney lysate (35 µg protein in RIPA buffer) stained with ARG64362 anti-HADH / HADHSC antibody at 0.02 µg/ml dilution.
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ARG64362 anti-HADH / HADHSC antibody WB image
Western Blot: HEK293 overexpressing HADH (RC201752) with C-terminal tag (DYKDDDDK) and stained with anti-DYKDDDDK in the left panel and with ARG64362 anti-HADH / HADHSC antibody in the right panel (mock transfection in first and last lanes)
