ARG22223
anti-HCN2 antibody [S71-37]
anti-HCN2 antibody [S71-37] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [S71-37] recognizes HCN2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Specificity | Detects ~95kDa. No cross-reactivity against HCN1. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S71-37 |
| Isotype | IgG1 |
| Target Name | HCN2 |
| Antigen Species | Rat |
| Immunogen | Fusion protein around aa. 761-863 of Rat HCN2 |
| Conjugation | Un-conjugated |
| Alternate Names | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2; HAC-1; BCNG2; Brain cyclic nucleotide-gated channel 2; BCNG-2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Hcn2 |
| Gene Full Name | hyperpolarization activated cyclic nucleotide-gated potassium channel 2 |
| Background | Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain.[supplied by OMIM, Jul 2010] |
| Function | Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Can also transport ammonium in the distal nephron. Produces a large instantaneous current. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages (By similarity). [UniProt] |
| Cellular Localization | Membrane |
| Calculated MW | 97 kDa |
| PTM | Phosphorylation at Ser-668 by PRKG2 shifts the voltage-dependence to more negative voltages, hence counteracting the stimulatory effect of cGMP on gating. |
Images (2) Click the Picture to Zoom In
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ARG22223 anti-HCN2 antibody [S71-37] WB image
Western blot: Rat brain membrane lysate stained with ARG22223 anti-HCN2 Antibody [S71-37] at 1:1000 dilution.
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ARG22223 anti-HCN2 antibody [S71-37] IHC-Fr image
Immunohistochemistry: 10% Formalin (12-24 hours at RT) fixed Mouse frozen brain section stained with ARG22223 anti-HCN2 Antibody [S71-37] (brown) at 1:1000 dilution (1 hour). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500uL for 5 minutes at RT.
