ARG63017
anti-HLA E antibody [MEM-E/02]
anti-HLA E antibody [MEM-E/02] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Primates
Immune System antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [MEM-E/02] recognizes HLA E |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | IHC-P, WB |
| Specificity | The clone MEM-E/02 specifically reacts with denaturated heavy chain of human HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of all human cell types. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-E/02 |
| Isotype | IgG1 |
| Target Name | HLA E |
| Antigen Species | Human |
| Immunogen | Recombinant human HLA-E denaturated heavy chain. |
| Conjugation | Un-conjugated |
| Alternate Names | MHC class I antigen E; QA1; EA2.1; HLA-6.2; EA1.2; MHC; HLA class I histocompatibility antigen, alpha chain E |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Pretreatment: Heat antigen retrieval (sodium citrate). Incubation: 1 hour at RT; detection DAB. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | IHC-P: Human placenta |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P13747 Human HLA class I histocompatibility antigen, alpha chain E |
|---|---|
| Gene Symbol | HLA-E |
| Gene Full Name | major histocompatibility complex, class I, E |
| Background | HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| Function | Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 40 kDa |
Images (1) Click the Picture to Zoom In
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ARG63017 anti-HLA E antibody [MEM-E/02] IHC-P image
Immunohistochemistry: Colorectal epithelial cells between the four groups: normal mucosa (Negative expression), adenoma (Negative expression), early cancer group (weak positive) and advanced cancer group (strong positive) stained with ARG63017 anti-HLA E antibody [MEM-E/02].
From Renxiang Huang et al. Oncol Lett (2017), doi: 10.3892/ol.2017.5891, Fig. 5.
Specific References
Loss of Fas expression and high expression of HLA-E promoting the immune escape of early colorectal cancer cells
IHC-P, WB / Human
