ARG57547
anti-HPRT1 antibody [1D9]
anti-HPRT1 antibody [1D9] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [1D9] recognizes HPRT1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1D9 |
| Isotype | IgG2b, kappa |
| Target Name | HPRT1 |
| Antigen Species | Human |
| Immunogen | Recombinant Human HPRT (aa. 1-218) purified from E. coli. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.4.2.8; Hypoxanthine-guanine phosphoribosyltransferase; HGPRTase; HGPRT; HPRT |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P00492 Human Hypoxanthine-guanine phosphoribosyltransferase |
|---|---|
| Gene Symbol | HPRT1 |
| Gene Full Name | hypoxanthine phosphoribosyltransferase 1 |
| Background | The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009] |
| Function | Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. [UniProt] |
| Calculated MW | 25 kDa |
Images (1) Click the Picture to Zoom In
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ARG57547 anti-HPRT antibody [1D9] WB image
Western blot: 40 µg of HeLa, MCF7, 293T and A549 cell lysates stained with ARG57547 anti-HPRT antibody [1D9] at 1:1000 dilution.
