Overview

Product Description	Rabbit Polyclonal antibody recognizes HPRT1
Tested Reactivity	Hu, Ms, Rat
Tested Application	IHC-P, IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	HPRT1
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human HPRT1.
Conjugation	Un-conjugated
Alternate Names	EC 2.4.2.8; Hypoxanthine-guanine phosphoribosyltransferase; HGPRTase; HGPRT; HPRT

Application Instructions

Application Suggestion	Tested Application Dilution IHC-P 1:50 - 1:200 IP 1:50 WB 1:1000 - 1:5000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size	25 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 24465 Rat HPRT1 GeneID: 3251 Human HPRT1 Swiss-port # P00492 Human Hypoxanthine-guanine phosphoribosyltransferase Swiss-port # P27605 Rat Hypoxanthine-guanine phosphoribosyltransferase
Gene Symbol	HPRT1
Gene Full Name	hypoxanthine phosphoribosyltransferase 1
Background	The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]
Function	Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. [UniProt]
Cellular Localization	Cytoplasm. [UniProt]
Calculated MW	25 kDa

Images (1) Click the Picture to Zoom In