ARG44488
anti-HSD3B7 antibody
anti-HSD3B7 antibody for ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes HSD3B7 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HSD3B7 |
| Antigen Species | Human |
| Immunogen | Human HSD3B7 recombinant protein |
| Conjugation | Un-conjugated |
| Alternate Names | HSD3B7; Hydroxy-Delta-5-Steroid Dehydrogenase, 3 Beta- And Steroid Delta-Isomerase 7; C(27)-3BETA-HSD; SDR11E3; Short Chain Dehydrogenase/Reductase Family 11E, Member 3; Cholest-5-Ene-3-Beta,7-Alpha-Diol 3-Beta-Dehydrogenase; 3-Beta-Hydroxy-Delta(5)-C27 Steroid Oxidoreductase; 3 Beta-Hydroxysteroid Dehydrogenase Type VII; 3 Beta-Hydroxysteroid Dehydrogenase Type 7; C(27) 3-Beta-HSD; 3-Beta-HSD VII; 3 Beta-Hydroxy-Delta 5-C27-Steroid Oxidoreductase |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Bioinformation
| Database Links |
Swiss-port # Q9H2F3 Human 3 beta-hydroxysteroid dehydrogenase type 7 |
|---|---|
| Gene Symbol | HSD3B7 |
| Gene Full Name | Hydroxy-Delta-5-Steroid Dehydrogenase, 3 Beta- And Steroid Delta-Isomerase 7 |
| Background | This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Function | The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis. |
| Cellular Localization | Endoplasmic reticulum, Membrane |
| Calculated MW | 41 kDa |
Images (2) Click the Picture to Zoom In
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ARG44488 anti-HSD3B7 antibody ICC/IF image
Immunofluorescence: A549 stained with ARG44488 anti-HSD3B7 antibody at 5 μg/mL dilution.
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ARG44488 anti-HSD3B7 antibody WB image
Western blot: HeLa, MCF-7 and HepG2 stained with ARG44488 anti-HSD3B7 antibody at 0.5 μg/mL dilution.
