ARG58810
anti-HSF2 antibody
anti-HSF2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes HSF2 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Ms, Hm |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HSF2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 82-102 of Human HSF2 (KQERDGPVEFQHPYFKQGQDD). |
| Conjugation | Un-conjugated |
| Alternate Names | HSF 2; Heat shock factor protein 2; Heat shock transcription factor 2; HSTF 2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSF2 |
| Gene Full Name | heat shock transcription factor 2 |
| Background | The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011] |
| Function | DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasmic during normal growth and moves to the nucleus upon activation. [UniProt] |
| Calculated MW | 60 kDa |
Images (5) Click the Picture to Zoom In
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ARG58810 anti-HSF2 antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG58810 anti-HSF2 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG58810 anti-HSF2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG58810 anti-HSF2 antibody.
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ARG58810 anti-HSF2 antibody WB image
Western blot: 50 µg of Rat kidney, 50 µg of Rat Spleen, 40 µg of 293T, 40 µg of MCF-7, 40 µg of Jurkat, 40 µg of A549 and 40 µg of CEM lysates stained with ARG58810 anti-HSF2 antibody at 0.5 µg/ml dilution.
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ARG58810 anti-HSF2 antibody FACS image
Flow Cytometry: 293T cells were blocked with 10% normal goat serum and then stained with ARG58810 anti-HSF2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG58810 anti-HSF2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG58810 anti-HSF2 antibody.
