ARG45305
anti-HUCE1 antibody
anti-HUCE1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Polyclonal antibody recognizes HUCE1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HUCE1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human HUCE1. |
| Conjugation | Un-conjugated |
| Alternate Names | RRP8; Ribosomal RNA Processing 8; KIAA0409; NML; Nucleomethylin; Ribosomal RNA-Processing Protein 8; Cerebral Protein 1; Ribosomal RNA Processing 8, Methyltransferase, Homolog (Yeast); Ribosomal RNA Processing 8, Methyltransferase, Homolog; RRP8 Methyltransferase Homolog (S. Cerevisiae); RRP8 Methyltransferase Homolog; EC 2.1.1.- |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 65 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RRP8 |
| Gene Full Name | Ribosomal RNA Processing 8 |
| Background | Enables methylated histone binding activity. Involved in several processes, including cellular response to glucose starvation; intrinsic apoptotic signaling pathway by p53 class mediator; and regulation of gene expression. Acts upstream of with a negative effect on regulation of G1 to G0 transition. Located in several cellular components, including cytosol; nuclear lumen; and rDNA heterochromatin. Part of eNoSc complex. [provided by Alliance of Genome Resources, Nov 2024] |
| Function | Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 51 kDa |
| PTM | Phosphoprotein. [UniProt] |
