ARG56954
anti-Hsp 105 antibody [J1G12]
anti-Hsp 105 antibody [J1G12] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [J1G12] recognizes Hsp 105 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | J1G12 |
| Isotype | IgG2b, kappa |
| Target Name | Hsp 105 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-858 of Human Hsp 105. |
| Conjugation | Un-conjugated |
| Alternate Names | HSP105B; HSP105A; Heat shock protein 105 kDa; HSP105; Heat shock 110 kDa protein; NY-CO-25; Antigen NY-CO-25 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSPH1 |
| Gene Full Name | heat shock 105kDa/110kDa protein 1 |
| Function | Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (By similarity). [UniProt] |
| Calculated MW | 97 kDa |
| PTM | Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity. |
Images (2) Click the Picture to Zoom In
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ARG56954 anti-Hsp 105 antibody [J1G12] FACS image
Flow Cytometry: Hep3B cell line stained with ARG56954 anti-Hsp 105 antibody [J1G12] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG56954 anti-Hsp 105 antibody [J1G12] WB image
Western blot: 40 µg of 1) HeLa, 2) HepG2, 3) NIH-3T3, 4) MCF7, 5) U-87 MG, and 6) A549 cell lysates stained with ARG56954 anti-Hsp 105 antibody [J1G12] at 1:1000.
