ARG59815
anti-IARS / Isoleucyl tRNA synthetase antibody
anti-IARS / Isoleucyl tRNA synthetase antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IARS / Isoleucyl tRNA synthetase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IARS / Isoleucyl tRNA synthetase |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1158-1262 of Human IARS (NP_002152.2). |
| Conjugation | Un-conjugated |
| Alternate Names | ILERS; IARS1; EC 6.1.1.5; IleRS; IRS; ILRS; Isoleucyl-tRNA synthetase; Isoleucine--tRNA ligase, cytoplasmic; PRO0785 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Jurkat, HL-60 and HepG2 | ||||
| Observed Size | 160 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P41252 Human Isoleucine--tRNA ligase, cytoplasmic |
|---|---|
| Gene Symbol | IARS |
| Gene Full Name | isoleucyl-tRNA synthetase |
| Background | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. [provided by RefSeq, Nov 2012] |
| Cellular Localization | Cytoplasm. Cytoplasm, cytosol. [UniProt] |
| Calculated MW | 144 kDa |
Images (1) Click the Picture to Zoom In
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ARG59815 anti-IARS / Isoleucyl tRNA synthetase antibody WB image
Western blot: 25 µg of Jurkat, HL-60 and HepG2 cell lysates stained with ARG59815 anti-IARS / Isoleucyl tRNA synthetase antibody at 1:1000 dilution.
