ARG54401
anti-ICAD antibody
anti-ICAD antibody for ICC/IF,Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ICAD |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Specificity | This antibody recognizes non-cleaved (45kDa) and cleaved DFF45. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ICAD |
| Antigen Species | Human |
| Immunogen | Peptide corresponding to aa 313-331 at the C-terminus of human DFF45 (accession no. NP_004392). |
| Conjugation | Un-conjugated |
| Alternate Names | DFF-45; DNA fragmentation factor 45 kDa subunit; Inhibitor of CAD; ICAD; DFF1; DNA fragmentation factor subunit alpha |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa, Jurkat, A431 and K562 |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunoaffinity chroma-tography |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O00273 Human DNA fragmentation factor subunit alpha |
|---|---|
| Gene Symbol | DFFA |
| Gene Full Name | DNA fragmentation factor, 45kDa, alpha polypeptide |
| Background | A human DNA fragmentation factor (DFF) which is cleaved by caspase-3 during apoptosis was identified recently. The mouse homologue of human DFF was identified as a DNase inhibitor and was designated ICAD. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in nuclei. Cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is a hallmark of apoptotic cell death. |
| Function | Inhibitor of the caspase-activated DNase (DFF40). [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody |
| Calculated MW | 37 kDa |
| PTM | Caspase-3 cleaves DFF45 at 2 sites to generate an active factor. |
Images (2) Click the Picture to Zoom In
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ARG54401 anti-ICAD antibody WB image
Western blot: H:HeLaJ:Jurkat; A:A431; K:K562 stained with ARG54401 anti-ICAD antibody at 1 μg/ml dilution.
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ARG54401 anti-ICAD antibody ICC/IF image
Immunofluorescence: HeLa stained with ARG54401 anti-ICAD antibody at 5 μg/ml dilution.
