ARG65699
anti-IDH1 antibody
anti-IDH1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Publication2
Overview
| Product Description | Mouse Monoclonal antibody recognizes IDH1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | IDH1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to a region of Human IDH1. |
| Conjugation | Un-conjugated |
| Alternate Names | IDPC; EC 1.1.1.42; Cytosolic NADP-isocitrate dehydrogenase; IDP; HEL-S-26; HEL-216; Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; PICD; IDCD; NADP; Oxalosuccinate decarboxylase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | MCF-7, HepG2, HeLa, A549, A431, Jurkat, human brain tissue, human liver tissue, human kidney tissue, human colon carcinoma tissue, human breast tissue. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | 1*TBS (pH 7.4), 0.05% Sodium azide, 1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 1% BSA, 40% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IDH1 |
| Gene Full Name | isocitrate dehydrogenase 1 (NADP+), soluble |
| Background | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013] |
| Highlight | Related products: Isocitrate Dehydrogenase antibodies; Isocitrate Dehydrogenase ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: TCA intermediate fumarate promotes mitobiogenesis |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 47 kDa |
| PTM | Acetylation at Lys-374 dramatically reduces catalytic activity. |
Images (2) Click the Picture to Zoom In
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ARG65699 anti-IDH1 antibody WB image
Western blot: 30 µg of 1) Mouse brain, and 2) Rat brain lysates stained with ARG65699 anti-IDH1 antibody at 1:1000 dilution.
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ARG65699 anti-IDH1 antibody WB image
Western blot: 30 µg of U87 cell lysate stained with ARG65699 anti-IDH1 antibody at 1:1000 dilution.
Customer's Feedback
Excellent
Review for anti-IDH1 antibody
Application：WB
Sample：U87
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-IDH1 antibody
Application：WB
Sample：1) Mouse brain, 2) Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
The effect of ochratoxin A on cytotoxicity and glucose metabolism in human esophageal epithelium Het-1A cells
WB / Human
Ochratoxin A induces reprogramming of glucose metabolism by switching energy metabolism from oxidative phosphorylation to glycolysis in human gastric epithelium GES-1 cells in vitro.
WB / Human
