ARG54644
anti-IGF1 antibody [0.T.61]
anti-IGF1 antibody [0.T.61] for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Neutralizing,Western blot and Mouse,Rat
Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [0.T.61] recognizes IGF1 |
|---|---|
| Tested Reactivity | Ms, Rat |
| Predict Reactivity | Chk |
| Tested Application | IHC-P, IP, Neut, WB |
| Specificity | Recognizes human Insulin-Like Growth Factor I (IGF1), Mr ~17kD (migrates to ~7kD). Crossreactivity with IGF-II is ~40%. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 0.T.61 |
| Isotype | IgG1, kappa |
| Target Name | IGF1 |
| Antigen Species | Human |
| Immunogen | Human insulin-like growth factor I (IGF1) purified from human plasma. |
| Conjugation | Un-conjugated |
| Alternate Names | MGF; Insulin-like growth factor I; Mechano growth factor; Somatomedin-C; IGFI; IGF-I |
Application Instructions
| Application Suggestion |
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| Application Note | Neut: 10-20 µg/ml can inhibit the activity of 10 ng/ml of IGF1 as determined by testing with chicken embryo fibroblasts (CEF) using an ATP endpoint assay. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | IHC-P: formalin-fixed, paraffin-embedded skin sections. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with Protein G. |
| Buffer | 0.1M Tris-glycine (pH 7.4), 0.15 mM sodium chloride and 0.1mM EDTA |
| Stabilizer | 0.1mM EDTA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IGF1 |
| Gene Full Name | insulin-like growth factor 1 (somatomedin C) |
| Background | The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009] |
| Function | The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. [UniProt] |
| Research Area | Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody |
| Calculated MW | 22 kDa |
