ARG66422
anti-IL38 antibody [SQab18124]
anti-IL38 antibody [SQab18124] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [SQab18124] recognizes IL38 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SQab18124 |
| Isotype | IgG2b, kappa |
| Target Name | IL38 |
| Antigen Species | Human |
| Immunogen | Recombinant Human IL38 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | IL1F10; Interleukin 1 Family Member 10; IL-1HY2; IL1-Theta; IL-1F10; FKSG75; Interleukin-1 Receptor Antagonist FKSG75; Interleukin-1 Family Member 10; Family Of Interleukin 1-Theta; Interleukin-1 HY2; Interleukin-38; FIL1 Theta; IL-1 Theta; MGC119832; MGC119833; MGC11983; IL1HY2; IL-38; Interleukin-1 Receptor Antagonist-Like FIL1 Theta; Interleukin 1 Family, Member 10 (Theta); Interleukin 1 Family Member 10 (Theta); IL-1F10 (Canonical Form IL-1F10a); Interleukin-1 Theta; FIL1- Theta; FIL1-Theta; FIL1T; IL38 |
Application Instructions
| Application Suggestion |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Purification | Affinity purification with immunogen. |
|---|---|
| Buffer | PBS (pH 7.4) and 0.01% Thimerosal. |
| Preservative | 0.01% Thimerosal |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL1F10 |
| Gene Full Name | interleukin 1 family, member 10 (theta) |
| Background | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses. Two alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008] |
| Function | Cytokine with immunomodulatory activity. Alone, does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. Reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. Increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS). Ligand for IL-36R/IL1RL2. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 17 kDa |
Images (4) Click the Picture to Zoom In
-
ARG66422 anti-IL38 antibody [SQab18124] ICC/IF image
Immunofluorescence: A549 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66422 anti-IL38 antibody [SQab18124] (green) at 1:200 dilution and cell nuclei were stained with Hoechst 33342 (blue).
-
ARG66422 anti-IL38 antibody [SQab18124] FACS image
Flow Cytometry: A549 cells were stained with ARG66422 anti-IL38 antibody [SQab18124] at 2 µg/ml (red) and without antibody control (black).
-
ARG66422 anti-IL38 antibody [SQab18124] WB image
Western blot: 50 ng of Recombinant IL38 protein, 30 µg of 293T and 30 µg of IL38 transfected 293T cell lysates stained with ARG66422 anti-IL38 antibody [SQab18124] at 1:5000 dilution.
-
ARG66422 anti-IL38 antibody [SQab18124] ELISA image
ELISA: Titration curve of ARG66422 anti-IL38 antibody [SQab18124]. Red: IL38; Black: BSA (negative control).
