ARG63232
anti-IRF2 antibody
anti-IRF2 antibody for Western blot and Human
Gene Regulation antibody; Immune System antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes IRF2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IRF2 |
| Antigen Species | Human |
| Immunogen | KTSDITQARVKSC |
| Conjugation | Un-conjugated |
| Alternate Names | IRF-2; Interferon regulatory factor 2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody; Immune System antibody |
| Calculated MW | 39 kDa |
| PTM | Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity. The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation with SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter. |
Images (2) Click the Picture to Zoom In
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ARG63232 anti-IRF2 antibody WB image
Western Blot: Jurkat lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63232 anti-IRF2 antibody at 2 µg/ml dilution.
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ARG63232 anti-IRF2 antibody WB image
Western Blot: 1). Mock transfection; 2) IRF2 (RC202102) expressing plasmid transfected HEK293 cell lysate standed with ARG63232 anti-IRF2 antibody
