ARG53951
anti-IgM antibody [CH2] (APC)
anti-IgM antibody [CH2] (APC) for Flow cytometry and Human
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Allophycocyanin-conjugated Mouse Monoclonal antibody [CH2] recognizes IgM |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The clone CH2 reacts with Fc fragment of human IgM. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CH2 |
| Isotype | IgG1 |
| Target Name | IgM |
| Antigen Species | Human |
| Immunogen | Purified human IgM. |
| Conjugation | Allophycocyanin |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography . |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days. |
|---|---|
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody |
Images (3) Click the Picture to Zoom In
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ARG53951 anti-IgM antibody [CH2] (APC) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG53951 anti-IgM antibody [CH2] (APC) at 0.6 µg/ml dilution.
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ARG53951 anti-IgM antibody [CH2] (APC) FACS image
Flow Cytometry: Human lymphocytes stained with ARG53951 anti-IgM antibody [CH2] (APC) at 0.6 µg/ml dilution and ARG53783 anti-CD19 antibody [LT19] (PE) (20 µl reagent / 100 µl of peripheral whole blood).
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ARG53951 anti-IgM antibody [CH2] (APC) FACS image
Flow Cytometry: Separation of human IgM positive B cells (red-filled) from IgM negative CD19 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG53951 anti-IgM antibody [CH2] (APC) at 0.6 µg/ml dilution.
