ARG65466
anti-Insulin antibody [IN-05] (low endotoxin)
anti-Insulin antibody [IN-05] (low endotoxin) for ELISA,Functional study,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Radioimmunoassay and Human,Cow,Pig
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [IN-05] recognizes Insulin |
|---|---|
| Tested Reactivity | Hu, Cow, Pig |
| Tested Application | ELISA, FuncSt, ICC/IF, IHC-P, RIA |
| Specificity | The antibody IN-05 reacts with insulin, one of the major regulatory endocrine hormones of intermediate metabolism, normally secreted by the beta cells (a type of islet cells) of the pancreas; it is also present in tumors of B cell origin such as insulinoma. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | IN-05 |
| Isotype | IgG1 |
| Target Name | Insulin |
| Antigen Species | Pig |
| Immunogen | Porcine insulin. |
| Conjugation | Un-conjugated |
| Alternate Names | IDDM; IDDM2; IDDM1; ILPR; MODY10; Insulin; IRDN |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein. |
| Buffer | PBS |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | INS |
| Gene Full Name | insulin |
| Background | This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2019] |
| Function | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 12 kDa |
Clone References
Transforming growth factor beta is a critical regulator of adult human islet plasticity.
IHC-P / Human
Murine hybridoma monoclonal antibodies against insulin: cross-reactivity with insulins of three species and blocking of insulin binding to its receptor.
