ARG62524
anti-Involucrin antibody [SY5]
anti-Involucrin antibody [SY5] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Dog,Gorilla,Monkey,Owl,Pig
Cancer antibody; Signaling Transduction antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [SY5] recognizes Involucrin |
|---|---|
| Tested Reactivity | Hu, Dog, Glla, Mk, Owl, Pig |
| Species Does Not React With | Ms |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | This antibody stains involucrin in different sizes: 120 kDa in human keratinocytes, 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog (weak band) and a doublet of 105kDa in pig. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SY5 |
| Isotype | IgG1 |
| Target Name | Involucrin |
| Antigen Species | Human |
| Immunogen | Pure involucrin from human keratinocytes |
| Epitope | between codon 421-568 of human involucrin |
| Conjugation | Un-conjugated |
| Alternate Names | Involucrin |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunogen affinity purified |
| Buffer | 1X PBS buffer with < 0.1% sodium azide. |
| Preservative | < 0.1% sodium azide. |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IVL |
| Gene Full Name | involucrin |
| Background | Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008] |
| Function | Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. [UniProt] |
| Cellular Localization | Cytoplasm |
| Research Area | Cancer antibody; Signaling Transduction antibody |
| Calculated MW | 68 kDa |
| PTM | Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. |
Images (1) Click the Picture to Zoom In
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ARG62524 anti-Involucrin antibody [SY5] WB image
Western blot: HaCaT stained with ARG62524 anti-Involucrin antibody [SY5] at 1:1000 dilution.
From Chun-Yin Yang et al. Antioxidants (Basel). (2022), doi: 10.3390/antiox11112304, Fig. 7.
Specific References
Antioxidant, Anti-Inflammation and Antiaging Activities of Artocarpus altilis Methanolic Extract on Urban Particulate Matter-Induced HaCaT Keratinocytes Damage
WB / Human
