ARG65852
anti-JunB antibody
anti-JunB antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes JunB |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | Recognizes endogenous levels of JunB protein. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | JunB |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide around the C-terminus of Human JunB. |
| Conjugation | Un-conjugated |
| Alternate Names | AP-1; Transcription factor jun-B |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Sodium citrate buffer (10mM, pH 6.0), Boiling bathing for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | Liquid (pH 7.3), 0.42% Potassium phosphate, 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | JUNB |
| Gene Full Name | jun B proto-oncogene |
| Function | Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. [UniProt] |
| Calculated MW | 36 kDa |
| PTM | Ubiquitinated by ITCH, leading to its degradation. |
Images (3) Click the Picture to Zoom In
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ARG65852 anti-JunB antibody ICC/IF image
Immunofluorescence: Formalin-fixed HEK293 cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 min and blocked with 3% BSA-PBS for 30 min at RT. Cells were stained with ARG65852 anti-JunB antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at RT in the dark. DAPI was used to stain the cell nuclei (blue).
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ARG65852 anti-JunB antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer tissue section. Antigen retrieval: Sodium citrate buffer (10mM, pH 6.0), boiling bathing for 20 min. The section was stained with ARG65852 anti-JunB antibody at RT and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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ARG65852 anti-JunB antibody WB image
Western blot: MCF7 whole cell lysates stained with ARG65852 anti-JunB antibody.
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Review for anti-JunB antibody
Application：WB
Sample：MCF7
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Ac-SDKP Attenuates Activation of Lung Macrophages and Bone Osteoclasts in Rats Exposed to Silica by Inhibition of TLR4 and RANKL Signaling Pathways.
WB / Rat
