ARG66265
anti-K-Ras antibody
anti-K-Ras antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes K-Ras |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | WB |
| Specificity | The immunogen sequence was designed to react K-Ras (Isoform 2A) only and it does not react to H-Ras and N-Ras tested by ELISA. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | K-Ras |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of Human K-Ras. |
| Conjugation | Un-conjugated |
| Alternate Names | NS3; KRAS1; KRAS2; RALD; K-RAS4A; GTPase KRas; c-Ki-ras; KI-RAS; RASK2; K-RAS2B; K-RAS2A; K-RAS4B; c-K-ras; C-K-RAS; NS; CFC2; Ki-Ras; K-Ras 2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | 22 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRAS |
| Gene Full Name | Kirsten rat sarcoma viral oncogene homolog |
| Background | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008] |
| Function | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. [UniProt] |
| Calculated MW | 22 kDa |
| PTM | Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG66265 anti-K-Ras antibody WB image
Western blot: 293T cell lysate stained with ARG66265 anti-K-Ras antibody at 1:500 dilution.
