ARG40886
anti-KCC2 / Potassium Chloride Cotransporter phospho (Thr1007) antibody
anti-KCC2 / Potassium Chloride Cotransporter phospho (Thr1007) antibody for Western blot and Human,Mouse,Rat,Bovine,Primates,Pig,Sheep
Overview
| Product Description | Rabbit Polyclonal antibody recognizes KCC2 / Potassium Chloride Cotransporter phospho (Thr1007) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, NHuPrm, Pig, Sheep |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | KCC2 / Potassium Chloride Cotransporter |
| Antigen Species | Mouse |
| Immunogen | Phosphospecific peptide around Thr1007 of Mouse KCC2 / Potassium Chloride Cotransporter. |
| Conjugation | Un-conjugated |
| Alternate Names | K-Cl cotransporter 2; Electroneutral potassium-chloride cotransporter 2; Solute carrier family 12 member 5; hKCC2; KCC2; Neuronal K-Cl cotransporter |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | 135 kDa |
Properties
| Form | Liquid |
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| Purification | Affinity purification with phospho-specific peptide and the non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 50% Glycerol and 100 µg/ml BSA. |
| Stabilizer | 50% Glycerol and 100 µg/ml BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | SLC12A5 |
| Gene Full Name | solute carrier family 12 (potassium/chloride transporter), member 5 |
| Background | K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008] |
| Function | Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons. [UniProt] |
| Cellular Localization | Membrane; Multi-pass membrane protein. [UniProt] |
| Calculated MW | 126 kDa |
Images (1) Click the Picture to Zoom In
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ARG40886 anti-KCC2 / Potassium Chloride Cotransporter phospho (Thr1007) antibody WB image
Western blot: Rat hippocampus untreated or treated with lambda phosphatase (1200 units for 30 min). The blots were stained with ARG40886 anti-KCC2 / Potassium Chloride Cotransporter phospho (Thr1007) antibody.
