ARG40323
anti-KCNE1 antibody
anti-KCNE1 antibody for Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes KCNE1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | KCNE1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 1-100 of Human KCNE1 (NP_000210.2). |
| Conjugation | Un-conjugated |
| Alternate Names | LQT5; MinK; JLNS2; Delayed rectifier potassium channel subunit IsK; Minimal potassium channel; ISK; LQT2/5; Potassium voltage-gated channel subfamily E member 1; IKs producing slow voltage-gated potassium channel subunit beta Mink; JLNS |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Mouse brain and U937 | ||||
| Observed Size | 15 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P15382 Human Potassium voltage-gated channel subfamily E member 1 Swiss-port # P23299 Mouse Potassium voltage-gated channel subfamily E member 1 |
|---|---|
| Gene Symbol | KCNE1 |
| Gene Full Name | potassium channel, voltage gated subfamily E regulatory beta subunit 1 |
| Background | The product of this gene belongs to the potassium channel KCNE family. Potassium ion channels are essential to many cellular functions and show a high degree of diversity, varying in their electrophysiologic and pharmacologic properties. This gene encodes a transmembrane protein known to associate with the product of the KVLQT1 gene to form the delayed rectifier potassium channel. Mutation in this gene are associated with both Jervell and Lange-Nielsen and Romano-Ward forms of long-QT syndrome. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008] |
| Function | Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. Assembled with KCNQ1/KVLQT1 is proposed to form the slowly activating delayed rectifier cardiac potassium (IKs) channel. The outward current reaches its steady state only after 50 seconds. Assembled with KCNH2/HERG may modulate the rapidly activating component of the delayed rectifying potassium current in heart (IKr). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Apical cell membrane. Membrane raft. Note=Colocalizes with KCNB1 at the plasma membrane (By similarity). Targets to the membrane raft when associated with KNCQ1 (PubMed:20533308). [UniProt] |
| Calculated MW | 15 kDa |
| PTM | Phosphorylation inhibits the potassium current. N-glycosylation at Asn-26 occurs post-translationally, and requires prior cotranslational glycosylation at Asn-5. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG40323 anti-KCNE1 antibody WB image
Western blot: 25 µg of Mouse brain and U937 cell lysates stained with ARG40323 anti-KCNE1 antibody at 1:1000 dilution.
