Overview

Product Description	Rabbit Polyclonal antibody recognizes KDM1B / LSD2
Tested Reactivity	Hu, Ms, Rat
Tested Application	FACS, ICC/IF, IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	KDM1B / LSD2
Antigen Species	Human
Immunogen	Synthetic peptide of Human KDM1B / LSD2.
Conjugation	Un-conjugated
Alternate Names	dJ298J15.2; Lysine-specific histone demethylase 1B; Flavin-containing amine oxidase domain-containing protein 1; C6orf193; EC 1.-.-.-; Lysine-specific histone demethylase 2; AOF1; LSD2; bA204B7.3

Application Instructions

Application Suggestion	Tested Application Dilution FACS 1:50 ICC/IF 1:50 - 1:200 IP 1:60 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HeLa
Observed Size	~ 88 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 218214 Mouse KDM1B GeneID: 221656 Human KDM1B Swiss-port # Q8CIG3 Mouse Lysine-specific histone demethylase 1B Swiss-port # Q8NB78 Human Lysine-specific histone demethylase 1B
Gene Symbol	KDM1B
Gene Full Name	lysine (K)-specific demethylase 1B
Background	Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 [PubMed 19407342]).[supplied by OMIM, Oct 2009]
Function	Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (By similarity). [UniProt]
Cellular Localization	Nucleus. [UniProt]
Calculated MW	92 kDa

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