ARG56303
anti-Lipoamide Dehydrogenase antibody
anti-Lipoamide Dehydrogenase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Lipoamide Dehydrogenase |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Lipoamide Dehydrogenase |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human Lipoamide Dehydrogenase |
| Conjugation | Un-conjugated |
| Alternate Names | DLDH; LAD; Dihydrolipoamide dehydrogenase; DLDD; PHE3; EC 1.8.1.4; Glycine cleavage system L protein; Dihydrolipoyl dehydrogenase, mitochondrial; GCSL; E3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | BT474 |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O08749 Mouse Dihydrolipoyl dehydrogenase, mitochondrial Swiss-port # P09622 Human Dihydrolipoyl dehydrogenase, mitochondrial |
|---|---|
| Gene Symbol | DLD |
| Gene Full Name | dihydrolipoamide dehydrogenase |
| Background | This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Function | Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction. [UniProt] |
| Calculated MW | 54 kDa |
| PTM | Tyrosine phosphorylated. |
Images (2) Click the Picture to Zoom In
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ARG56303 anti-Lipoamide Dehydrogenase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver injury stained with ARG56303 anti-Lipoamide Dehydrogenase antibody at 1:100 dilution.
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ARG56303 anti-Lipoamide Dehydrogenase antibody WB image
Western blot: BT474 cell lysate stained with ARG56303 anti-Lipoamide Dehydrogenase antibody.
