ARG66009
anti-M-CSF antibody (Biotin)
anti-M-CSF antibody (Biotin) for ELISA,Western blot and Human
Overview
| Product Description | Biotin-conjugated Rabbit Polyclonal antibody recognizes M-CSF |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | M-CSF |
| Antigen Species | Human |
| Immunogen | E. coli derived recombinant Human M-CSF. (MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS) |
| Conjugation | Biotin |
| Alternate Names | Macrophage colony-stimulating factor 1; CSF-1; Lanimostim; M-CSF; MCSF |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by affinity chromatography. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P09603 Human Macrophage colony-stimulating factor 1 |
|---|---|
| Gene Symbol | CSF1 |
| Gene Full Name | colony stimulating factor 1 (macrophage) |
| Background | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011] |
| Function | Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [UniProt] |
| Calculated MW | 60 kDa |
| PTM | N- and O-glycosylated. Glycosylation and proteolytic cleavage yield different soluble forms. One high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1 is N- and O-glycosylated. Isoform 3 is only N-glycosylated. |
Images (4) Click the Picture to Zoom In
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ARG66009 anti-M-CSF antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Human M-CSF stained with ARG66009 anti-M-CSF antibody (Biotin), under non-reducing conditions.
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ARG66009 anti-M-CSF antibody (Biotin) standard curve image
Direct ELISA: ARG66009 anti-M-CSF antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG66009 anti-M-CSF antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Human M-CSF stained with ARG66009 anti-M-CSF antibody (Biotin), under reducing conditions.
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ARG66009 anti-M-CSF antibody (Biotin) standard curve image
Sandwich ELISA: ARG66009 anti-M-CSF antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG66008 anti-M-CSF antibody as a capture antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
