ARG58117
anti-MAD1 antibody
anti-MAD1 antibody for ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MAD1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MAD1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human MAD1. |
| Epitope | Recombinant funsion protein corresponding to aa. 360-700 of Human MAD1 (NP_001013859.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Mitotic spindle assembly checkpoint protein MAD1; Tax-binding protein 181; MAD1-like protein 1; MAD1; Mitotic checkpoint MAD1 protein homolog; hMAD1; HsMAD1; TP53I9; PIG9; TXBP181; Mitotic arrest deficient 1-like protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa | ||||||
| Observed Size | 95 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9Y6D9 Human Mitotic spindle assembly checkpoint protein MAD1 |
|---|---|
| Gene Symbol | MAD1L1 |
| Gene Full Name | MAD1 mitotic arrest deficient-like 1 (yeast) |
| Background | MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
| Function | Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding. [UniProt] |
| Cellular Localization | Chromosome, Cytoplasm, Nucleus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle. [UniProt] |
| Calculated MW | 83 kDa |
| PTM | Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG58117 anti-MAD1 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG58117 anti-MAD1 antibody.
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ARG58117 anti-MAD1 antibody WB image
Western blot: 25 µg of HeLa cell lysate stained with ARG58117 anti-MAD1 antibody at 1:1000 dilution.
