ARG41197
anti-MAFB antibody
anti-MAFB antibody for Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MAFB |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MAFB |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 184-323 of Human MAFB (NP_005452.2). |
| Conjugation | Un-conjugated |
| Alternate Names | Transcription factor MafB; Maf-B; MCTO; KRML; V-maf musculoaponeurotic fibrosarcoma oncogene homolog B |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Rat liver | ||||
| Observed Size | 50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MAFB |
| Gene Full Name | v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog B |
| Background | The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns. [provided by RefSeq, Jul 2008] |
| Function | Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter (By similarity). Involved either as an oncogene or as a tumor suppressor, depending on the cell context. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 36 kDa |
| PTM | Phosphorylated by GSK3 and MAPK13 on serine and threonine residues. Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors (By similarity). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG41197 anti-MAFB antibody WB image
Western blot: 25 µg of Rat liver lysate stained with ARG41197 anti-MAFB antibody at 1:1000 dilution.
