ARG54031
anti-MAP2 antibody (N-term)
anti-MAP2 antibody (N-term) for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse
Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes MAP2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Target Name | MAP2 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human MAP2 (N-terminus) protein fragments expressed in E.coli |
| Conjugation | Un-conjugated |
| Alternate Names | MAP2A; Microtubule-associated protein 2; MAP2C; MAP2B; MAP-2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 300 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.1M Tris-Glycine (pH 7.4), 150mM NaCl, 0.2%Sodium azide, 0.1%BSA and 50%Glycerol |
| Preservative | 0.2%Sodium azide |
| Stabilizer | 0.1%BSA, 50%Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MAP2 |
| Gene Full Name | microtubule-associated protein 2 |
| Background | The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization.They also seem to have a stiffening effect on microtubules. |
| Function | The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. [UniProt] |
| Cellular Localization | Cytoplasm, cytoskeleton |
| Highlight | Related products: MAP2 antibodies; MAP2 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Neuronal Development Marker Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| Research Area | Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody |
| Calculated MW | 200 kDa |
| PTM | Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity). |
Images (3) Click the Picture to Zoom In
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ARG54031 anti-MAP2 antibody (N-term) ICC/IF image
Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG54031 anti-MAP2 antibody (N-term) at 1:100 dilution.
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ARG54031 anti-MAP2 antibody (N-term) WB image
Western blot: Mouse brain lysate stained with ARG54031 anti-MAP2 antibody (N-term) at 1:1000 dilution.
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ARG54031 anti-MAP2 antibody (N-term) IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54031 anti-MAP2 antibody (N-term).
Customer's Feedback
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Review for anti-MAP2 antibody (N-term)
Application：WB
Sample：Mouse brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
