ARG44569
anti-MED28 / EG1 antibody
anti-MED28 / EG1 antibody for Flow cytometry,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MED28 / EG1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MED28 / EG1 |
| Antigen Species | Human |
| Immunogen | Human MED28 / EG1 recombinant protein (aa. sequence: M1-T178). |
| Conjugation | Un-conjugated |
| Alternate Names | MED28; Mediator Complex Subunit 28; Magicin 2; EG1; Mediator Of RNA Polymerase II Transcription Subunit 28; Merlin And Grb2-Interacting Cytoskeletal Protein; Tumor Angiogenesis Marker EG-1; Endothelial-Derived Protein 1; DKFZP434N185; Mediator Of RNA Polymerase II Transcription, Subunit 28 Homolog (S. Cerevisiae); Mediator Of RNA Polymerase II Transcription, Subunit 28 Homolog; Endothelial-Derived Gene 1; 1500003D12Rik |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Preservative | 4% Trehalose |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9H204 Human Mediator of RNA polymerase II transcription subunit 28 |
|---|---|
| Gene Symbol | MED28 |
| Gene Full Name | Mediator Complex Subunit 28 |
| Background | Predicted to enable actin binding activity. Predicted to act upstream of or within negative regulation of smooth muscle cell differentiation and stem cell population maintenance. Located in nucleoplasm. [provided by Alliance of Genome Resources, Apr 2022] |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways. [Uniprot] |
| Cellular Localization | Cytoplasm, Membrane, Nucleus. [Uniprot] |
| Calculated MW | 20 kDa |
Images (1) Click the Picture to Zoom In
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ARG44569 anti-MED28 / EG1 antibody WB image
Western blot: K562 and HepG2 stained with ARG44569 anti-MED28 / EG1 antibody at 0.5 μg/mL dilution.
