ARG45114
anti-MED4 antibody
anti-MED4 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MED4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MED4 |
| Antigen Species | Human |
| Immunogen | E.coli-derived human MED4 recombinant protein |
| Conjugation | Un-conjugated |
| Alternate Names | MED4 ; Mediator Complex Subunit 4; DRIP36; HSPC126; TRAP36; VDRIP; Vitamin D3 Receptor-Interacting Protein Complex 36 KDa Component ; Mediator Of RNA Polymerase II Transcription Subunit 4; Activator-Recruited Cofactor 36 KDa Component; ARC36 ; Mediator Of RNA Polymerase II Transcription, Subunit 4 Homolog (S. Cerevisiae); Vitamin D Receptor-Interacting Protein, 36-KD; Vitamin D Receptor Interacting Protein; TRAP/SMCC/PC2 Subunit P36 Subunit; Mediator, 34-KD Subunit, Homolog |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MED4 |
| Gene Full Name | Mediator Complex Subunit 4 |
| Background | This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. [Unuprot] |
| Cellular Localization | Nucleus. [Unuprot] |
| Calculated MW | 30 kDa |
| PTM | Acetylation, Phosphoprotein. [Unuprot] |
Images (2) Click the Picture to Zoom In
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ARG45114 anti-MED4 antibody ICC/IF image
Immunofluorescence: HeLa stained with ARG45114 anti-MED4 antibody at 5 μg/ml dilution.
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ARG45114 anti-MED4 antibody WB image
Western blot: ANA-1 stained with ARG45114 anti-MED4 antibody at 0.25 μg/ml dilution.
