ARG59395
anti-MED4 antibody
anti-MED4 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MED4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MED4 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-270 of Human MED4 (NP_054885.1). |
| Conjugation | Un-conjugated |
| Alternate Names | HSPC126; Vitamin D3 receptor-interacting protein complex 36 kDa component; DRIP36; TRAP/SMCC/PC2 subunit p36 subunit; Mediator of RNA polymerase II transcription subunit 4; VDRIP; Activator-recruited cofactor 36 kDa component; TRAP36; ARC36; Mediator complex subunit 4 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Jurkat | ||||
| Observed Size | 37 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NPJ6 Human Mediator of RNA polymerase II transcription subunit 4 |
|---|---|
| Gene Symbol | MED4 |
| Gene Full Name | mediator complex subunit 4 |
| Background | This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 30 kDa |
Images (1) Click the Picture to Zoom In
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ARG59395 anti-MED4 antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG59395 anti-MED4 antibody at 1:1000 dilution.
