ARG41162
anti-MGEA5 / OGA antibody
anti-MGEA5 / OGA antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MGEA5 / OGA |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MGEA5 / OGA |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 380-580 of Human MGEA5 (NP_001135906.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; N-acetyl-beta-D-glucosaminidase; MEA5; NCOAT; EC 3.2.1.-; Meningioma-expressed antigen 5; Beta-N-acetylhexosaminidase; Beta-hexosaminidase; EC 3.2.1.169; Protein O-GlcNAcase; Beta-N-acetylglucosaminidase; OGA; N-acetyl-beta-glucosaminidase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse brain | ||||||
| Observed Size | ~120-130 kda |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MGEA5 |
| Gene Full Name | meningioma expressed antigen 5 (hyaluronidase) |
| Background | The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008] |
| Function | Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1. [UniProt] |
| Cellular Localization | Isoform 3: Nucleus. Isoform 1: Cytoplasm. [UniProt] |
| Calculated MW | 103 kDa |
| PTM | Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG41162 anti-MGEA5 / OGA antibody WB image
Western blot: 25 µg of Mouse brain lysate stained with ARG41162 anti-MGEA5 / OGA antibody at 1:1000 dilution.
