ARG22664
anti-MHC Class I H-2b + D + P + Q + w16 antibody [ER-HR52]
anti-MHC Class I H-2b + D + P + Q + w16 antibody [ER-HR52] for Flow cytometry,IHC-Frozen sections and Mouse
Overview
| Product Description | Rat Monoclonal antibody [ER-HR52] recognizes MHC Class I H-2b + D + P + Q + w16 This antibody recognizes a polymorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells. Clone ER-HR52 specifically recognizes mouse strains with the haplotypes H-2b, w16 and H-2d, p, q. Mouse strains with the haplotypes H-2f, r, s, w17, w23, w27 show weak reactivity with this antibody. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, IHC-Fr |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | ER-HR52 |
| Isotype | IgG2a |
| Target Name | MHC Class I H-2b + D + P + Q + w16 |
| Antigen Species | Mouse |
| Immunogen | Adherent F1 (CBAxBL) bone marrow stormal cells. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
