ARG45909
anti-MID2 / TRIM1 antibody
anti-MID2 / TRIM1 antibody for Flow cytometry,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MID2 / TRIM1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MID2 / TRIM1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to middle region of human MID2 / TRIM1. |
| Conjugation | Un-conjugated |
| Alternate Names | Midline 2; RNF60; FXY2; RING finger protein 60; Probable E3 ubiquitin-protein ligase MID2; EC 6.3.2.-; Midline defect 2; MRX101; Midline-2; TRIM1; Tripartite motif-containing protein 1; Midin-2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 83 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity chromatography purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9QUS6 Mouse Probable E3 ubiquitin-protein ligase MID2 Swiss-port # Q9UJV3 Human Probable E3 ubiquitin-protein ligase MID2 |
|---|---|
| Gene Symbol | MID2 |
| Gene Full Name | Midline 2 |
| Background | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009] |
| Function | E3 ubiquitin ligase that plays a role in microtubule stabilization. Mediates the 'Lys-48'-linked polyubiquitination of LRRK2 to drive its localization to microtubules and its proteasomal degradation in neurons. This ubiquitination inhibits LRRK2 kinase activation by RAB29 [UniProt] |
| Cellular Localization | Cytoplasm; Cytoskeleton; Microtubule. [UniProt] |
| Calculated MW | 83 kDa |
| PTM | Phosphorylated on serine and threonine residues. [UniProt] |
