ARG42199

anti-MOB4 antibody

anti-MOB4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes MOB4
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MOB4
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-225 of Human MOB4 (NP_056202.2).
Conjugation Un-conjugated
Alternate Names Preimplantation protein 3; 2C4D; Mob3; PHOCN; CGI-95; Mps one binder kinase activator-like 3; PREI3; MOB-like protein phocein; MOB3; Class II mMOB1; MOB1; MOBKL3; Mob1 homolog 3

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MCF7
Observed Size ~ 23 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 171050 Rat MOB4

GeneID: 19070 Mouse MOB4

GeneID: 25843 Human MOB4

Gene Symbol MOB4
Gene Full Name MOB family member 4, phocein
Background This gene was identified based on its similarity with the mouse counterpart. Studies of the mouse counterpart suggest that the expression of this gene may be regulated during oocyte maturation and preimplantation following zygotic gene activation. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Naturally occurring read-through transcription occurs between this locus and the neighboring locus HSPE1. [provided by RefSeq, Feb 2011]
Function May play a role in membrane trafficking, specifically in membrane budding reactions. [UniProt]
Cellular Localization Cytoplasm, perinuclear region. Membrane; Peripheral membrane protein. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Note=In a perinuclear punctate pattern. Associated with membranes and the Golgi stacks. [UniProt]
Calculated MW 26 kDa
PTM Phosphorylated on serine residues. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG42199 anti-MOB4 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat ovary tissue stained with ARG42199 anti-MOB4 antibody at 1:100 dilution.

  • ARG42199 anti-MOB4 antibody WB image

    Western blot: 25 µg of MCF7 cell lysate stained with ARG42199 anti-MOB4 antibody at 1:3000 dilution.