ARG42173
anti-MOGAT2 antibody
anti-MOGAT2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes MOGAT2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow, Dog, Pig |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MOGAT2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human MOGAT2. (C-KESAAHILNRK) (NP_079374.2) |
| Conjugation | Un-conjugated |
| Alternate Names | DGAT2L5; Acyl-CoA:monoacylglycerol acyltransferase 2; 2-acylglycerol O-acyltransferase 2; Diacylglycerol O-acyltransferase candidate 5; Diacylglycerol acyltransferase 2-like protein 5; MGAT2; hDC5; hMGAT2; EC 2.3.1.22; Monoacylglycerol O-acyltransferase 2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Human duodenum | ||||||
| Observed Size | ~ 37 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q3SYC2 Human 2-acylglycerol O-acyltransferase 2 |
|---|---|
| Gene Symbol | MOGAT2 |
| Gene Full Name | monoacylglycerol O-acyltransferase 2 |
| Background | The protein encoded by this gene is an enzyme that catalyzes the synthesis of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. The encoded protein is important in the uptake of dietary fat by the small intestine. This protein forms a complex with diacylglycerol O-acyltransferase 2 in the endoplasmic reticulum, and this complex catalyzes the synthesis of triacylglycerol. [provided by RefSeq, Dec 2015] |
| Function | Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity. [UniProt] |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. [UniProt] |
| Calculated MW | 38 kDa |
Images (2) Click the Picture to Zoom In
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ARG42173 anti-MOGAT2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42173 anti-MOGAT2 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG42173 anti-MOGAT2 antibody WB image
Western blot: 35 µg of Human duodenum lysate (in RIPA buffer) stained with ARG42173 anti-MOGAT2 antibody at 0.05 µg/ml dilution and incubated at RT for 1 hour.
