ARG58359
anti-MRPL45 antibody
anti-MRPL45 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MRPL45 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MRPL45 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 57-306 of Human MRPL45 (NP_115727.5). |
| Conjugation | Un-conjugated |
| Alternate Names | L45mt; MRP-L45 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | U-87MG | ||||||||
| Observed Size | 35 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9BRJ2 Human 39S ribosomal protein L45, mitochondrial Swiss-port # Q9D0Q7 Mouse 39S ribosomal protein L45, mitochondrial |
|---|---|
| Gene Symbol | MRPL45 |
| Gene Full Name | mitochondrial ribosomal protein L45 |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q. [provided by RefSeq, May 2013] |
| Cellular Localization | Mitochondrion. [UniProt] |
| Calculated MW | 35 kDa |
Images (1) Click the Picture to Zoom In
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ARG58359 anti-MRPL45 antibody WB image
Western blot: 25 µg of U-87MG cell lysate stained with ARG58359 anti-MRPL45 antibody at 1:3000 dilution.
