ARG57021
anti-MRPS25 antibody [38E7]
anti-MRPS25 antibody [38E7] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [38E7] recognizes MRPS25 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 38E7 |
| Isotype | IgG2a, kappa |
| Target Name | MRPS25 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-173 of Human MRPS25. |
| Conjugation | Un-conjugated |
| Alternate Names | S25mt; RPMS25; MRP-S25; 28S ribosomal protein S25, mitochondrial |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P82663 Human 28S ribosomal protein S25, mitochondrial |
|---|---|
| Gene Symbol | MRPS25 |
| Gene Full Name | mitochondrial ribosomal protein S25 |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. [provided by RefSeq, Jul 2008] |
| Calculated MW | 20 kDa |
Images (1) Click the Picture to Zoom In
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ARG57021 anti-MRPS25 antibody [38E7] WB image
Western blot: 40 µg of HepG2 cell lysate stained with ARG57021 anti-MRPS25 antibody [38E7] at 1) 1:500, 2) 1:1000, and, 3) 1:5000 dilution.
