ARG59023
anti-MXD1 / Mad antibody
anti-MXD1 / Mad antibody for Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MXD1 / Mad |
|---|---|
| Tested Reactivity | Ms, Rat |
| Predict Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MXD1 / Mad |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to M1-L221 of Human Mad. |
| Conjugation | Un-conjugated |
| Alternate Names | Protein MAD; BHLHC58; MAD1; Max dimerization protein 1; MAD; Max dimerizer 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | MXD1 |
|---|---|
| Gene Full Name | MAX dimerization protein 1 |
| Background | This gene encodes a member of the MYC/MAX/MAD network of basic helix-loop-helix leucine zipper transcription factors. The MYC/MAX/MAD transcription factors mediate cellular proliferation, differentiation and apoptosis. The encoded protein antagonizes MYC-mediated transcriptional activation of target genes by competing for the binding partner MAX and recruiting repressor complexes containing histone deacetylases. Mutations in this gene may play a role in acute leukemia, and the encoded protein is a potential tumor suppressor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011] |
| Function | Transcriptional repressor. MAD binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MAD thus antagonizes MYC transcriptional activity by competing for MAX. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 25 kDa |
| PTM | Ubiquitinated by BIRC2/c-IAP1, leading to its subsequent degradation by the proteasome. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG59023 anti-MXD1 / Mad antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat testis and Mouse ovary lysates stained with ARG59023 anti-MXD1 / Mad antibody at 0.5 µg/ml, overnight at 4°C.
