ARG59443
anti-NDRG3 antibody
anti-NDRG3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDRG3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDRG3 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to L181-R239 of Human NDRG3. |
| Conjugation | Un-conjugated |
| Alternate Names | N-myc downstream-regulated gene 3 protein; Protein NDRG3 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NDRG3 |
| Gene Full Name | NDRG family member 3 |
| Calculated MW | 41 kDa |
Images (5) Click the Picture to Zoom In
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ARG59443 anti-NDRG3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain tissues. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59443 anti-NDRG3 antibody at 1 µg/ml, overnight at 4°C.
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ARG59443 anti-NDRG3 antibody WB image
Western blot: 50 µg of sample under reducing conditions. COLO320 whole cell lysate stained with ARG59443 anti-NDRG3 antibody at 0.5 µg/ml, overnight at 4°C.
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ARG59443 anti-NDRG3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissues. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59443 anti-NDRG3 antibody at 1 µg/ml, overnight at 4°C.
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ARG59443 anti-NDRG3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissues. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59443 anti-NDRG3 antibody at 1 µg/ml, overnight at 4°C.
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ARG59443 anti-NDRG3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59443 anti-NDRG3 antibody at 1 µg/ml, overnight at 4°C.
